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Transesterification of a Tertiary Alcohol by Engineered Candida antarctica Lipase A
Author(s) -
Löfgren Johanna,
Görbe Tamás,
Oschmann Michael,
Svedendahl Humble Maria,
Bäckvall JanE.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800792
Subject(s) - candida antarctica , transesterification , lipase , chemistry , steric effects , enzyme , alcohol , biocatalysis , organic chemistry , protein engineering , stereochemistry , solvent , directed evolution , catalysis , biochemistry , reaction mechanism , mutant , gene
Tertiary alcohols are known to be challenging substrates for applications in asymmetric synthesis due to their complexity and steric hinderance. The occurrence of tertiary alcohols and their esters in nature indicates the presence of natural biocatalytic synthetic routes for their preparation. Lipase A from Candida antarctica (CalA) is a hydrolase that has previously been shown to catalyze the transesterification of racemic 2‐phenylbut‐3‐yn‐2‐ol at a low rate. In this work, the activity of that enzyme was improved by protein engineering through a semi‐rational design strategy. An enzyme library was created and screened for transesterification activity towards racemic 2‐phenylbut‐3‐yn‐2‐ol in an organic solvent. One successful enzyme variant (L367G) showed a tenfold increased reaction rate compared to the wild‐type enzyme, while maintaining a high enantioselectivity.