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Live Imaging and Quantitation of Lipid Droplets and Mitochondrial Membrane Potential Changes with Aggregation‐Induced Emission Luminogens in an in Vitro Model of Liver Steatosis
Author(s) -
Zhou Yabin,
Hua Jin,
Barritt Greg,
Liu Yong,
Tang Ben Zhong,
Tang Youhong
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800765
Subject(s) - steatosis , amiodarone , fluorescence , in vitro , chemistry , lipid droplet , biophysics , membrane potential , mitochondrion , membrane , biochemistry , chromatography , biology , medicine , endocrinology , physics , quantum mechanics , atrial fibrillation
High specificity, low background, good biocompatibility and photostability are common properties of aggregation‐induced emission luminogens (AIEgens). In this study, an AIEgen FAS was used in live HepG2 cells, an in vitro model of liver steatosis, to quantify lipid droplet number and size instead of the traditional method of only measuring fluorescence intensity emitted from fluorescence dye stained in lipid droplet. In parallel, another AIEgen, TPE‐Ph‐In, was used to perform continuous monitoring and quantitation of mitochondrial membrane potential in the same batch of live HepG2 cells. The data show a significant increase in lipid droplet numbers after 24 h treatment by amiodarone and a significant increase in both lipid droplet numbers and size after 48 h amiodarone treatment. Moreover, the data suggest a significant increase in mitochondria membrane potential in cells treated with amiodarone for 24 and 48 h, with restoration to pre‐treatment level 24 h after removal of the amiodarone. Further investigation is needed to fully understand the underlying mechanism.