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Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)
Author(s) -
Palei Shubhendu,
Becher Kira S.,
Nienberg Christian,
Jose Joachim,
Mootz Henning D.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800748
Subject(s) - cell sorting , cyclic peptide , peptide , chemistry , computational biology , combinatorial chemistry , biochemistry , cell , biology
The cover feature picture shows a modular connector approach for presenting semisynthetic cyclic peptides on the cell surface of E. coli . Cyclization is achieved upon addition of a synthetic peptide to live cells expressing a recombinant fragment through protein trans‐splicing and oxime ligation. The mild reaction conditions preserve cellular viability and allow sorting by fluorescent‐activated cell sorting (FACS). This unnatural bacterial‐display format holds potential for applications in drug discovery by directed evolution of diverse peptide macrocycles. More information can be found in the communication by H. D. Mootz et al. on page 72 in Issue 1, 2019 (DOI: 10.1002/cbic.201800552).