Premium
Colloidal Fluorophore Aggregates for the Selective Detection of Albumins in Solution and on Electrophoresis Gels
Author(s) -
Park Jihee,
Kim Youngmi
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800608
Subject(s) - fluorophore , human serum albumin , fluorescence , chemistry , reagent , chromatography , electrophoresis , serum albumin , human blood , albumin , biochemistry , biology , organic chemistry , physiology , physics , quantum mechanics
We report a fluorescent probe that is highly sensitive and selective for serum albumins. Signal transduction results from the disassembly of fluorescence‐quenched aggregates upon binding to human serum albumin (HSA). The probe offers a rapid (≤2 s) fluorometric assay of HSA in phosphate‐buffered saline, urine, or blood serum with a high signal‐to‐noise ratio. It is also useful as a wash‐free prestaining reagent for detecting HSA on electrophoresis gels.