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Gene Discovery in Gelsemium Highlights Conserved Gene Clusters in Monoterpene Indole Alkaloid Biosynthesis
Author(s) -
Franke Jakob,
Kim Jeongwoon,
Hamilton John P.,
Zhao Dongyan,
Pham Gina M.,
WiegertRininger Krystle,
Crisovan Emily,
Newton Linsey,
Vaillancourt Brieanne,
Tatsis Evangelos,
Buell C. Robin,
O'Connor Sarah E.
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800592
Subject(s) - monoterpene , indole alkaloid , indole test , biosynthesis , gene , alkaloid , biology , stereochemistry , botany , chemistry , biochemistry
Genome mining is a routine technique in microbes for discovering biosynthetic pathways. In plants, however, genomic information is not commonly used to identify novel biosynthesis genes. Here, we present the genome of the medicinal plant and oxindole monoterpene indole alkaloid (MIA) producer Gelsemium sempervirens (Gelsemiaceae). A gene cluster from Catharanthus roseus , which is utilized at least six enzymatic steps downstream from the last common intermediate shared between the two plant alkaloid types, is found in G. sempervirens , although the corresponding enzymes act on entirely different substrates. This study provides insights into the common genomic context of MIA pathways and is an important milestone in the further elucidation of the Gelsemium oxindole alkaloid pathway.