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Cover Feature: Quick Click: The DNA‐Templated Ligation of 3′‐ O ‐Propargyl‐ and 5′‐Azide‐Modified Strands Is as Rapid as and More Selective than Ligase (ChemBioChem 19/2018)
Author(s) -
Osman Eiman A.,
Gadzikwa Tendai,
Gibbs Julianne M.
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800545
Subject(s) - click chemistry , azide , cycloaddition , propargyl , dna ligase , oligonucleotide , combinatorial chemistry , chemistry , dna , biochemistry , organic chemistry , catalysis
The cover feature picture shows how DNA‐templated, copper‐mediated azide–alkyne cycloaddition (CUAAC) with 3′‐ O ‐propargyl and 5′‐azide‐terminated oligonucleotides is extremely rapid, reaching completion within minutes. Not only is the reaction fast, but also highly selective when compared with T4 DNA ligase. At temperatures between the dissociation temperatures of the matched and the single base pair–mismatched systems, excellent selectivities are observed for CUAAC that are superior to that from the enzyme. More information can be found in the full paper by J. M. Gibbs et al. on page 2081 in Issue 19, 2018 (DOI: 10.1002/cbic.201800305). Cover art by Shyam Parshotam.