Premium
Cell‐Compatible Nanoprobes for Imaging Intracellular Phosphatase Activities
Author(s) -
Wang Jiaqing,
Zhou Jie,
He Hongjian,
Wu Difei,
Du Xuewen,
Xu Bing
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800495
Subject(s) - intracellular , phosphatase , quinazolinone , live cell imaging , cell , microbiology and biotechnology , molecular imaging , biochemistry , chemistry , enzyme , biology , biophysics , combinatorial chemistry , genetics , in vivo
Phosphatases play an important role in cell biology, but only a few probes are suitable for selectively imaging phosphatase activity in live cells, because the current probes require cell fixation or exhibit considerable cytotoxicity. Herein, we show that conjugating a d ‐peptide to a quinazolinone derivative generates cell‐compatible, biostable probes for imaging the phosphatase activity inside live cells. Moreover, our results show that inhibiting ectophosphatases is a critical factor for imaging intracellular phosphatases. As the first example of using selective inhibitors to ensure intracellular function of molecular probes, this work illustrates a facile approach to design molecular probes for profiling the activities of enzymes in a spatial, selective manner in a complicated environment.