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Front Cover: Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions (ChemBioChem 17/2018)
Author(s) -
NagyGyőr László,
Abaházi Emese,
Bódai Viktória,
Sátorhelyi Péter,
Erdélyi Balázs,
BaloghWeiser Diána,
Paizs Csaba,
Hornyánszky Gábor,
Poppe László
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800452
Subject(s) - biocatalysis , chemistry , amine gas treating , chromobacterium violaceum , cascade reaction , transaminase , cascade , organic chemistry , catalysis , combinatorial chemistry , chromatography , enzyme , biochemistry , reaction mechanism , quorum sensing , virulence , gene
The front cover picture shows a novel biocatalyst for cascade biotransformations comprising co‐immobilized cells with two different biocatalytic activities. A rational strategy based on an improved sol–gel process using hollow silica microspheres as a supporting additive was applied to co‐immobilize various whole cells with different biocatalytic activities directly after harvesting fermentations. Entrapment of Escherichia coli cells with Chromobacterium violaceum ω‐transaminase activity and Lodderomyces elongisporus cells with ketoreductase activity in presence of silica microspheres (depicted here as footballs) provided a biocatalyst with easy recovery, long‐term storability and good mechanical stability. The co‐immobilized whole‐cell biocatalyst could perform a reaction cascade converting racemic 4‐phenylbutan‐2‐amine or heptan‐2‐amine to a nearly equimolar mixture of enantiomerically pure ( R )‐amine and the corresponding ( S )‐alcohol even in continuous‐flow mode. More information can be found in the communication by G. Hornyánszky, L. Poppe, et al. on page 1845 in Issue 17, 2018 (DOI: 10.1002/cbic.201800286).