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An Enzyme‐Mediated Amplification Strategy Enables Detection of β‐Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β‐Lactam Resistance
Author(s) -
deBoer Tara R.,
Tarlton Nicole J.,
Yamaji Reina,
AdamsSapper Sheila,
Wu Tiffany Z.,
Maity Santanu,
Vesgesna Giri K.,
Sadlowski Corinne M.,
DePaola Peter,
Riley Lee W.,
Murthy Niren
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800443
Subject(s) - enzyme , microbiology and biotechnology , enzyme assay , chemistry , biochemistry , biology
Biochemical assays that can identify β‐lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β‐lactamase probes do not have the sensitivity needed to measure β‐lactam resistance directly from patient samples. Here, we report the development of an instrument‐free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β‐lactamase 40 000‐fold, compared to the standard β‐lactamase probe nitrocefin.