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Compact Conjugated Polymer Dots with Covalently Incorporated Metalloporphyrins for Hypoxia Bioimaging
Author(s) -
Fang Xiaofeng,
Ju Bo,
Liu Zhihe,
Wang Fei,
Xi Guan,
Sun Zezhou,
Chen Haobin,
Sui Changxiang,
Wang Mingxue,
Wu Changfeng
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800438
Subject(s) - hypoxia (environmental) , conjugated system , tumor hypoxia , porphyrin , polymer , in vivo , chemistry , biophysics , brightness , quantum dot , covalent bond , materials science , nanotechnology , photochemistry , oxygen , organic chemistry , medicine , biology , optics , surgery , microbiology and biotechnology , radiation therapy , physics
Hypoxia is closely related to multiple diseases, especially in tumors, which increases the aggressiveness and drug resistance of cancer cells. Precise hypoxia imaging is of great significance for cancer diagnosis and the evaluation of therapeutic effects. A kind of hydrophobic polymer (i.e., PFPtTFPP) as an imaging probe for hypoxia with fluorene as an energy donor and an oxygen‐sensitive Pt II porphyrin as an energy acceptor was developed. Compact polymer dots (Pdots) with a small size were prepared by nanoprecipitation. The PFPtTFPP Pdots showed excellent hypoxia sensing in solution with high sensitivity and full reversibility. The emission intensity, quantum yields, lifetime, and single‐particle brightness significantly increased under hypoxia conditions. Remarkably, hypoxia imaging in vitro and in vivo was realized, and a clear increase in brightness was observed under hypoxia conditions and in the tumor area. Excellent hypoxia imaging ability is beneficial to potential applications in cancer diagnosis.