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A Versatile and Convenient Synthesis of 34 S‐Labeled Phosphorothioate Oligonucleotides
Author(s) -
Stulz Rouven,
Meuller Johan,
Baždarević Dženita,
Wennberg Huldt Charlotte,
Strömberg Roger,
Andersson Shalini,
Dahlén Anders
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800417
Subject(s) - oligonucleotide , chemistry , disulfide bond , combinatorial chemistry , sulfur , oligonucleotide synthesis , isotope , stereochemistry , biochemistry , organic chemistry , dna , physics , quantum mechanics
A synthetic protocol for 34 S‐labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS‐based assay analysis. This was enabled by a highly efficient, two‐step, one‐pot synthesis of 34 S‐labeled phenylacetyl disulfide ( 34 S‐PADS), starting from 34 S‐enriched elemental sulfur ( 34 S 8 ). 34 S‐PADS was subsequently used for stable isotope labeling (SIL) of oligonucleotides containing a phosphorothioate backbone. The 34 S‐SIL PS ONs are shown to retain the same melting temperature, antisense activity, and secondary structure as those of the corresponding unlabeled 32 S PS ONs.