Premium
Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions
Author(s) -
NagyGyőr László,
Abaházi Emese,
Bódai Viktória,
Sátorhelyi Péter,
Erdélyi Balázs,
BaloghWeiser Diána,
Paizs Csaba,
Hornyánszky Gábor,
Poppe László
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800286
Subject(s) - amine gas treating , biocatalysis , chromobacterium violaceum , chemistry , transaminase , cascade , combinatorial chemistry , alcohol , catalysis , organic chemistry , chromatography , enzyme , biochemistry , reaction mechanism , quorum sensing , virulence , gene
Abstract An improved sol–gel process involving the use of hollow silica microspheres as a supporting additive was applied for the co‐immobilization of whole cells of Escherichia coli with Chromobacterium violaceum ω‐transaminase activity and Lodderomyces elongisporus with ketoreductase activity. The co‐immobilized cells with two different biocatalytic activities could perform a cascade of reactions to convert racemic 4‐phenylbutan‐2‐amine or heptan‐2‐amine into a nearly equimolar mixture of the corresponding enantiomerically pure R amine and S alcohol even in continuous‐flow mode. The novel co‐immobilized whole‐cell system proved to be an easy‐to‐store and durable biocatalyst.