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Osmium Tag for Post‐transcriptionally Modified RNA
Author(s) -
Debnath Turja Kanti,
Okamoto Akimitsu
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800274
Subject(s) - osmium , chemistry , nucleotide , transfer rna , rna , mass spectrometry , stereochemistry , biochemistry , gene , ruthenium , catalysis , chromatography
5‐Methylcytidine (m 5 C) and 5‐methyluridine (m 5 U) are highly abundant post‐transcriptionally modified nucleotides that are observed in various natural RNAs. Such nucleotides were labeled through a chemical approach, as both underwent oxidation at the C5=C6 double bond, leading to the formation of osmium–bipyridine complexes, which could be identified by mass spectrometry. This osmium tag made it possible to distinguished m 5 C and m 5 U from their isomers, 2′‐ O ‐methylcytidine and 2′‐ O ‐methyluridine, respectively. Queuosine and 2‐methylthio‐ N 6 ‐isopentenyladenosine in tRNA were also tagged through complex formation.