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Peptide Nucleic Acid Conjugated with Ruthenium‐Complex Stabilizing Double‐Duplex Invasion Complex Even under Physiological Conditions
Author(s) -
Hibino Masaki,
Aiba Yuichiro,
Watanabe Yoshihito,
Shoji Osami
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800256
Subject(s) - peptide nucleic acid , duplex (building) , dna , conjugate , nucleic acid , ruthenium , conjugated system , peptide , nucleic acid thermodynamics , chemistry , oligonucleotide , biophysics , biochemistry , combinatorial chemistry , biology , base sequence , organic chemistry , mathematical analysis , polymer , mathematics , catalysis
Peptide nucleic acid (PNA) can form a stable duplex with DNA, and, accordingly, directly recognize double‐stranded DNA through the formation of a double‐duplex invasion complex, wherein a pair of complementary PNA strands form two PNA/DNA duplexes. Because invasion does not require prior denaturation of DNA, PNA holds great potential for in cellulo or in vivo applications. To broaden the applicability of PNA invasion, we developed a new conjugate of PNA with a ruthenium complex. This Ru–PNA conjugate exhibits higher DNA‐binding affinity, which results in enhanced invasion efficiency, even under physiological conditions.