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Cover Feature: Protein–Polymer Microcapsules for PCR Technology (ChemBioChem 10/2018)
Author(s) -
Wang Xiaoliang,
Liu Yang,
Liu Jiao,
Chen Zhijun
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800206
Subject(s) - mcherry , polymer , fluorescence , biomolecule , chemistry , aqueous solution , dna , green fluorescent protein , nanotechnology , polymerase chain reaction , biophysics , materials science , biochemistry , biology , gene , organic chemistry , physics , quantum mechanics
The cover feature picture shows a polymerase chain reaction (PCR) carried out on an aqueous solution within mCherry‐based microcapsules that were surrounded by the oil phase. These protein–polymer hybrid microcapsules are heat‐resistant and thus suitable for PCR use. After multiple steps and cycles, the DNA template was amplified, and the products gave a green fluorescent signal upon UV irradiation, whereas the red fluorescence of mCherry faded away. In this setting, the reactant molecules can easily be introduced into the microcapsules. Moreover, no crosslinking or water–oil phase transition is necessary. This protein–polymer microcapsule PCR system has potential in various biological applications. More information can be found in the full paper by Z. Chen et al. on page 1044 in Issue 10, 2018 (DOI: 10.1002/cbic.201800080).