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Front Cover: Site‐Selective Functionalization of Flagellin by Steric Self‐Protection: A Strategy To Facilitate Flagellin as a Self‐Adjuvanting Carrier in Conjugate Vaccine (ChemBioChem 8/2018)
Author(s) -
Peng ChiJiun,
Chen HsiuLing,
Chiu ChengHsun,
Fang JimMin
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800137
Subject(s) - flagellin , chemistry , tlr5 , conjugate , steric effects , combinatorial chemistry , monomer , biophysics , stereochemistry , biochemistry , biology , polymer , organic chemistry , mathematical analysis , receptor , mathematics , toll like receptor , innate immune system , gene
The front cover picture shows a feasible strategy that makes use of a steric self‐protection effect for the site‐selective modification of a bacterial flagellin that can retain its adjuvant activity and act as a carrier protein of conjugate vaccine. At first, flagellin monomers undergo self‐assembly to form a compact filament in a highly concentrated sodium sulfate solution. The protruding D2 and D3 domains can be selectively decorated, for example, by adding azido group to the lysine residues, while the D0 and D1 domains for the TLR5 binding and activation are inside filament under the steric self‐protection. The site‐selectively modified filament can undergo depolymerization in phosphate buffer to give flagellin monomers that can act as antigenic self‐adjuvanting carriers for conjugation with bacterial polysaccharide haptens to facilitate the development of carbohydrate vaccines against various bacterial pathogens, including multidrug‐resistant bacteria.