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A Carbapenem‐Based Off–On Fluorescent Probe for Specific Detection of Metallo‐β‐Lactamase Activities
Author(s) -
Mao Wuyu,
Wang Yaqun,
Qian Xiana,
Xia Lingying,
Xie Hexin
Publication year - 2019
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800126
Subject(s) - serine , carbapenem , microbiology and biotechnology , bacteria , antibiotics , enzyme , chemistry , lactam , beta lactamase inhibitors , fluorescence , pathogenic bacteria , reagent , biology , combinatorial chemistry , biochemistry , stereochemistry , genetics , physics , quantum mechanics
Metallo‐β‐lactamase is one of the major clinical threats because this β‐lactam‐hydrolyzing enzyme confers significant resistance to most β‐lactam antibiotics, including carbapenems, among bacterial pathogens. Reported herein is a novel fluorogenic sensor for the specific detection of metallo‐β‐lactamase activities. This carbapenem‐based reagent exhibits excellent selectivity to metallo‐β‐lactamase over other serine‐β‐lactamases, including serine carbapenemases. The usefulness of this probe was further demonstrated in the rapid identification of metallo‐β‐lactamase‐expressing pathogenic bacteria.