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Mannose‐Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen‐Presenting Cells
Author(s) -
Straßburger David,
Stergiou Natascha,
Urschbach Moritz,
Yurugi Hajime,
Spitzer Daniel,
Schollmeyer Dieter,
Schmitt Edgar,
Besenius Pol
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800114
Subject(s) - ethylene glycol , supramolecular chemistry , supramolecular polymers , chemistry , mannose receptor , nanotechnology , mannose , biocompatibility , biophysics , materials science , combinatorial chemistry , biochemistry , biology , organic chemistry , molecule , in vitro , macrophage
A modular route to prepare functional self‐assembling dendritic peptide amphiphiles decorated with mannosides, to effectively target antigen‐presenting cells, such as macrophages, is reported. The monomeric building blocks were equipped with tetra(ethylene glycol)s (TEGs) or labeled with a Cy3 fluorescent probe. Experiments on the uptake of the multifunctional supramolecular particles into murine macrophages (Mφs) were monitored by confocal microscopy and fluorescence‐activated cell sorting. Mannose‐decorated supramolecular polymers trigger a significantly higher cellular uptake and distribution, relative to TEG carrying bare polymers. No cytotoxicity or negative impact on cytokine production of the treated Mφs was observed, which emphasized their biocompatibility. The modular nature of the multicomponent supramolecular polymer coassembly protocol is a promising platform to develop fully synthetic multifunctional vaccines, for example, in cancer immunotherapy.