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Protein–Polymer Microcapsules for PCR Technology
Author(s) -
Wang Xiaoliang,
Liu Yang,
Liu Jiao,
Chen Zhijun
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800080
Subject(s) - biomolecule , polymer , nanotechnology , covalent bond , amphiphile , chemistry , mcherry , smart polymer , poly(n isopropylacrylamide) , materials science , copolymer , organic chemistry , biochemistry , green fluorescent protein , gene
Protein–polymer microcapsules have attracted much attention, due to their special features and potential in biological use. How to make the most of this type of bio‐abiotic hybrid material is an intriguing question. Nevertheless, several unsatisfactory technical issues significantly limited the application of these materials. For instance, introducing various biomolecules and crosslinking for the capsules remains challenging and problematic. In this report, recombinant mCherry protein was covalently linked with poly( N ‐isopropylacrylamide) (PNIPAAm) to form amphiphilic protein–polymer conjugates, which assembled into microcapsules. These microcapsules are thermoresistant and can be used in the polymerase chain reaction (PCR). In this setting, the reactant molecules can be readily and easily introduced into the microcapsules, and crosslinking and water–oil phase transition are not necessary. This protein–polymer microcapsule PCR system has potential in various biological applications.