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Front Cover: Cell Factory Design and Optimization for the Stereoselective Synthesis of Polyhydroxylated Compounds (ChemBioChem 4/2018)
Author(s) -
Wiesinger Thomas,
Bayer Thomas,
Milker Sofia,
Mihovilovic Marko D.,
Rudroff Florian
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800042
Subject(s) - aldolase a , aldol reaction , stereoselectivity , chemistry , adduct , yield (engineering) , biocatalysis , synthetic biology , front cover , dihydroxyacetone , alcohol dehydrogenase , combinatorial chemistry , stereochemistry , organic chemistry , alcohol , enzyme , cover (algebra) , biology , catalysis , computational biology , materials science , reaction mechanism , mechanical engineering , glycerol , engineering , metallurgy
The front cover picture shows an artificial metabolic mini‐pathway composed of an alcohol dehydrogenase and a dihydroxyacetone‐dependent aldolase for the synthesis of chiral aldol adducts in living Escherichia coli cells. Starting from primary alcohols, we designed and optimized this synthetic cascade on the genetic and on the down‐stream processing levels. The combination resulted in the synthesis of various chiral diols in up to 90% yield over two reaction steps. More information can be found in the full paper by F. Rudroff et al. on page 361 in Issue 4, 2018 (DOI: 10.1002/cbic.201700464).