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Microarray Analysis of Oligosaccharide‐Mediated Multivalent Carbohydrate–Protein Interactions and Their Heterogeneity
Author(s) -
Gade Madhuri,
Alex Catherine,
Leviatan BenArye Shani,
Monteiro João T.,
Yehuda Sharon,
Lepenies Bernd,
PadlerKaravani Vered,
Kikkeri Raghavendra
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800037
Subject(s) - oligosaccharide , epitope , computational biology , chemistry , carbohydrate , biochemistry , mannose , galactose , biology , antigen , genetics
Carbohydrate–protein interactions (CPIs) are involved in a wide range of biological phenomena. Hence, the characterization and presentation of carbohydrate epitopes that closely mimic the natural environment is one of the long‐term goals of glycosciences. Inspired by the multivalency, heterogeneity and nature of carbohydrate ligand‐mediated interactions, we constructed a combinatorial library of mannose and galactose homo‐ and hetero‐glycodendrons to study CPIs. Microarray analysis of these glycodendrons with a wide range of biologically important plant and animal lectins revealed that oligosaccharide structures and heterogeneity interact with each other to alter binding preferences.