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Cover Feature: Epitope‐Resolved Detection of Peanut‐Specific IgE Antibodies by Surface Plasmon Resonance Imaging (ChemBioChem 3/2018)
Author(s) -
Shen Min,
Joshi Amit A.,
Vannam Raghu,
Dixit Chandra K.,
Hamilton Robert G.,
Kumar Challa V.,
Rusling James F.,
Peczuh Mark W.
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700694
Subject(s) - surface plasmon resonance , epitope , chemistry , immunoglobulin e , epitope mapping , antibody , peptide , antigen , biophysics , nanotechnology , biochemistry , biology , immunology , materials science , nanoparticle
The cover feature picture shows the selective binding of IgE‐coated magnetic beads onto allergen‐functionalized array spots. Binding is detected by surface plasmon resonance imaging (SPRi), as depicted with the reflected red light below the array. Anti‐IgE antibodies and peptide and carbohydrate antigens from the peanut protein Ara h2 were selectively attached to individual array spots; this is shown by the different features on the gold dots. The beads were prepared by selective capture of IgEs from serum by IgE ϵ‐chain‐specific antibody‐coated particles, and differentiated in a microfluidic flow system. Only spots on the array containing specific allergenic epitopes bound the beads, and binding correlated with a clinical measure of peanut allergy. More information can be found in the communication by M. W. Peczuh et al. on page 199 in Issue 3, 2018 (DOI: 10.1002/cbic.201700513).