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Construction of T‐Motif‐Based DNA Nanostructures through Enzymatic Reactions
Author(s) -
Kageyama Ryo,
Kawamata Ibuki,
Tanabe Kaori,
Suzuki Yuki,
Nomura Shinichiro M.,
Murata Satoshi
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700682
Subject(s) - nanostructure , dna , dna origami , dna nanotechnology , motif (music) , enzyme , restriction enzyme , nanotechnology , annealing (glass) , chemistry , materials science , computational biology , biochemistry , biology , physics , acoustics , composite material
The most common way to fabricate DNA nanostructures is to mix individually synthesized DNA oligomers in one pot. However, if DNA nanostructures could be produced through enzymatic reactions, they could be applied in various environments, including in vivo. Herein, an enzymatic method developed to construct a DNA nanostructure from a simple motif called a T‐motif is reported. A long, repeated structure was replicated from a circular template by rolling circle amplification and then cleaved into T‐motif segments by restriction enzymes. These motifs have been successfully assembled into a ladder‐like nanostructure without purification or controlled annealing. This approach is widely applicable to constructing a variety of DNA nanostructures through enzymatic reactions.