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DNA Synthesis by Primer Exchange Reaction Cascades
Author(s) -
Hollenstein Marcel
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700639
Subject(s) - primer (cosmetics) , primer extension , dna , chemistry , dna synthesis , dna polymerase , dna sequencing , polymerase , computational biology , swap (finance) , combinatorial chemistry , synthetic biology , nanotechnology , biophysics , biology , base sequence , biochemistry , materials science , organic chemistry , finance , economics
Swap and extend : The autonomous synthesis of single‐stranded DNA molecules of arbitrary size and sequence composition can easily be achieved by primer exchange reaction (PER) cascades, in which the sequential polymerase‐mediated extension of DNA primers is guided by catalytic hairpins. This highlight illustrates the potential of this method for applications in DNA nanotechnology.