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Control of Protein Activity and Gene Expression by Cyclofen‐OH Uncaging
Author(s) -
Zhang Weiting,
Hamouri Fatima,
Feng Zhiping,
Aujard Isabelle,
Ducos Bertrand,
Ye Shixin,
Weiss Shimon,
Volovitch Michel,
Vriz Sophie,
Jullien Ludovic,
Bensimon David
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700630
Subject(s) - context (archaeology) , fusion protein , ligand (biochemistry) , chemistry , microbiology and biotechnology , gene , computational biology , biophysics , biochemistry , receptor , biology , recombinant dna , paleontology
The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light‐activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen‐OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.