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Alphanumerical Visual Display Made of DNA Logic Gates for Drug Susceptibility Testing of Pathogens
Author(s) -
Connelly Ryan P.,
Morozkin Evgeny S.,
Gerasimova Yulia V.
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700626
Subject(s) - alphanumeric , mycobacterium tuberculosis , dna , genome , computational biology , drug , biology , point mutation , genetics , mutation , tuberculosis , virology , gene , computer science , medicine , pharmacology , pathology , programming language
Molecular diagnostics of drug‐resistant pathogens require the analysis of point mutations in bacterial or viral genomes, which is usually performed by trained professionals and/or by sophisticated computer algorithms. We have developed a DNA‐based logic system that autonomously analyzes mutations found in the genome of Mycobacterium tuberculosis complex (MTC) bacteria and communicates the output to a human user as alphanumeric characters read by the naked eye. The five‐gate system displays “O” (“no infection”) for the absence of MTC infection and “P” or “F” for passing or failing a drug‐susceptibility test, respectively.