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MicroRNA Detection by DNA‐Mediated Liposome Fusion
Author(s) -
Jumeaux Coline,
Wahlsten Olov,
Block Stephan,
Kim Eunjung,
Chandrawati Rona,
Howes Philip D.,
Höök Fredrik,
Stevens Molly M.
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700592
Subject(s) - liposome , fusion , detection limit , computational biology , biosensor , payload (computing) , dna , lipid bilayer fusion , biophysics , nanotechnology , fusion gene , chemistry , biology , membrane , materials science , biochemistry , computer science , gene , chromatography , computer network , philosophy , linguistics , network packet
Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal‐generating molecules. In this work, sequence‐specific DNA‐mediated liposome fusion is used for the highly selective detection of microRNA. The detection of miR‐29a, a known flu biomarker, is demonstrated down to 18 n m within 30 min with high specificity by using a standard laboratory microplate reader. Furthermore, one order of magnitude improvement in the limit of detection is demonstrated by using a novel imaging technique combined with an intensity fluctuation analysis, which is coined two‐color fluorescence correlation microscopy.