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RNA‐Templated Concatenation of Triplet Nucleic‐Acid Probe
Author(s) -
Bahal Raman,
Manna Arunava,
Hsieh WeiChe,
Thadke Shivaji A.,
Sureshkumar Gopalsamy,
Ly Danith H.
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700574
Subject(s) - nucleic acid , concatenation (mathematics) , thioester , rna , cysteine , myotonic dystrophy , peptide nucleic acid , biochemistry , chemistry , native chemical ligation , nucleic acid structure , computational biology , combinatorial chemistry , biology , microbiology and biotechnology , gene , genetics , mathematics , combinatorics , enzyme
Template‐directed synthesis offers several distinct benefits over conventional laboratory creation, including unsurpassed reaction rate and selectivity. Although it is central to many biological processes, such an approach has rarely been applied to the in situ synthesis and recognition of biomedically relevant target. Towards this goal, we report the development of a three‐codon nucleic‐acid probe containing a C‐terminal thioester group and an N‐terminal cysteine that is capable of undergoing template‐directed oligomerization in the presence of an RNA target and self‐deactivation in its absence. The work has implications for the development of millamolecular nucleic‐acid probes for targeting RNA‐repeated expansions associated with myotonic dystrophy type 1 and other related neuromuscular and neurodegenerative disorders.