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Sustainable and Continuous Synthesis of Enantiopure l ‐Amino Acids by Using a Versatile Immobilised Multienzyme System
Author(s) -
VelascoLozano Susana,
da Silva Eunice S.,
Llop Jordi,
LópezGallego Fernando
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700493
Subject(s) - enantiopure drug , formate dehydrogenase , biocatalysis , amino acid , cationic polymerization , enantioselective synthesis , polymer , chemistry , agarose , formate , combinatorial chemistry , organic chemistry , chemical engineering , catalysis , chromatography , biochemistry , ionic liquid , engineering
The enzymatic synthesis of α‐amino acids is a sustainable and efficient alternative to chemical processes, through which achieving enantiopure products is difficult. To more address this synthesis efficiently, a hierarchical architecture that irreversibly co‐immobilises an amino acid dehydrogenase with polyethyleneimine on porous agarose beads has been designed and fabricated. The cationic polymer acts as an irreversible anchoring layer for the formate dehydrogenase. In this architecture, the two enzymes and polymer colocalise across the whole microstructure of the porous carrier. This multifunctional heterogeneous biocatalyst was kinetically characterised and applied to the enantioselective synthesis of a variety of canonical and noncanonical α‐amino acids in both discontinuous (batch) and continuous modes. The co‐immobilised bienzymatic system conserves more than 50 % of its initial effectiveness after five batch cycles and 8 days of continuous operation. Additionally, the environmental impact of this process has been semiquantitatively calculated and compared with the state of the art.