Premium
Native Chemical Ligation Directed by Photocleavable Peptide Nucleic Acid (PNA) Templates
Author(s) -
Middel Stephen,
Panse Cornelia H.,
Nawratil Swantje,
Diederichsen Ulf
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700487
Subject(s) - native chemical ligation , chemical ligation , ligation , linker , peptide nucleic acid , peptide , chemistry , nucleic acid , combinatorial chemistry , template , proximity ligation assay , covalent bond , sequencing by ligation , dna , biochemistry , chemical synthesis , nanotechnology , organic chemistry , biology , microbiology and biotechnology , computer science , base sequence , materials science , receptor , genomic library , in vitro , operating system
A novel peptide–peptide ligation strategy is introduced that has the potential to provide peptide libraries of linearly or branched coupled fragments and will be suited to introduce simultaneous protein modifications at different ligation sites. Ligation is assisted by templating peptide nucleic acid (PNA) strands, and therefore, ligation specificity is solely encoded by the PNA sequence. PNA templating, in general, allows for various kinds of covalent ligation reactions. As a proof of principle, a native chemical ligation strategy was elaborated. This PNA‐templated ligation includes easy on‐resin procedures to couple linkers and PNA to the respective peptides, and a traceless photocleavage of the linker/PNA oligomer after the ligation step. A 4,5‐dimethoxy‐2‐nitrobenzaldehyde‐based linker that allowed the photocleavable linkage of two bio‐oligomers was developed.