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Bioluminescence Resonance Energy Transfer (BRET)‐coupled Annexin V‐functionalized Quantum Dots for Near‐Infrared Optical Detection of Apoptotic Cells
Author(s) -
Tsuboi Setsuko,
Jin Takashi
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700486
Subject(s) - bioluminescence , autofluorescence , annexin , fluorescence , quantum dot , apoptosis , förster resonance energy transfer , biophysics , energy transfer , chemistry , cancer cell , materials science , nanotechnology , cancer , biology , physics , optics , biochemistry , genetics , molecular physics
Deregulation in apoptosis induces numerous diseases such as cancer, cardiovascular, and neurodegenerative diseases. Detection of apoptotic cells is crucial for understanding the mechanism of these diseases and for therapy development. Although optical imaging using visible‐emitting fluorescent probes, such as FITC‐labeled annexin V, is widely used for the detection of apoptotic cells, there are very limited probes that can be used in the near‐infrared region (NIR) over 700 nm. Compared with visible light, NIR light is highly permeable in turbid biological samples and tissues. In addition, optical imaging in the NIR region shows low autofluorescence from biological samples, leading to clearer images with high signal to background ratios. Here, we report the synthesis of bioluminescence resonance energy transfer (BRET)‐coupled annexin V‐functionalized quantum dots (QDs) and their application to NIR optical detection of apoptotic cells.