Premium
Labelling Proteins with Carbon Nanodots
Author(s) -
Rao Chethana,
Khan Syamantak,
Verma Navneet C.,
Nandi Chayan Kanti
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700440
Subject(s) - fluorescence , labelling , conjugated system , nanodot , chemistry , fluorescence spectroscopy , fluorescence correlation spectroscopy , photochemistry , carbon fibers , spectroscopy , absorption (acoustics) , chromophore , molecule , biophysics , materials science , biochemistry , organic chemistry , polymer , biology , physics , quantum mechanics , composite number , composite material
We present efficient labelling of several proteins with orange‐emissive carbon dots. N ‐Hydroxysuccinimide was used to activate the carboxyl groups of carbon dots, which subsequently reacted with the lysine groups present on the protein. Labelling was confirmed by UV absorption spectroscopy, PAGE and fluorescence correlation spectroscopy. Protein‐conjugated carbon dots showed an enhancement in fluorescence lifetime and intensity owing to reduced intramolecular dynamic fluctuations. Single‐molecule fluorescence measurements showed reduced fluorescence fluctuations and higher photon budget after protein tagging. Our study opens up opportunities to use carbon dots as highly precise biolabelling probes.