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Improved Enantioselectivity of Subtilisin Carlsberg towards Secondary Alcohols by Protein Engineering
Author(s) -
Dorau Robin,
Görbe Tamás,
Svedendahl Humble Maria
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700408
Subject(s) - subtilisin , protein engineering , chemistry , mutagenesis , site directed mutagenesis , directed evolution , substrate (aquarium) , catalysis , active site , stereochemistry , biocatalysis , organic chemistry , enzyme , combinatorial chemistry , biochemistry , mutation , mutant , reaction mechanism , biology , ecology , gene
Generally, the catalytic activity of subtilisin Carlsberg (SC) for transacylation reactions with secondary alcohols in organic solvent is low. Enzyme immobilization and protein engineering was performed to improve the enantioselectivity of SC towards secondary alcohols. Possible amino‐acid residues for mutagenesis were found by combining available literature data with molecular modeling. SC variants were created by site‐directed mutagenesis and were evaluated for a model transacylation reaction containing 1‐phenylethanol in THF. Variants showing high E values (>100) were found. However, the conversions were still low. A second mutation was made, and both the E values and conversions were increased. Relative to that shown by the wild type, the most successful variant, G165L/M221F, showed increased conversion (up to 36 %), enantioselectivity ( E values up to 400), substrate scope, and stability in THF.