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Back Cover: Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor‐Binding Element of Amine Transaminase (ChemBioChem 15/2017)
Author(s) -
Börner Tim,
Rämisch Sebastian,
Bartsch Sebastian,
Vogel Andreas,
Adlercreutz Patrick,
Grey Carl
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700373
Subject(s) - amination , cofactor , amine gas treating , transaminase , chemistry , enantioselective synthesis , protein engineering , catalysis , substrate (aquarium) , solvent , combinatorial chemistry , stereochemistry , organic chemistry , enzyme , biology , ecology
The back cover picture shows an engineered, highly stable amine transaminase that catalyses the enantioselective amination of ketones under process conditions, that is, high temperature and substrate concentration as well as solvent use. Through a semirational design, the two point mutations in the cofactor binding region (illustrated by the screws) prevent protein unfolding and cofactor loss (indicated by the chain) during catalysis. More information can be found in the communication by C. Grey et al. on page 1482 in Issue 15, 2017 (DOI: 10.1002/cbic.201700236).