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Inside Back Cover: Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity (ChemBioChem 14/2017)
Author(s) -
Babkova Petra,
Sebestova Eva,
Brezovsky Jan,
Chaloupkova Radka,
Damborsky Jiri
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700335
Subject(s) - extant taxon , robustness (evolution) , template , substrate specificity , cover (algebra) , sequence (biology) , biology , evolutionary biology , chemistry , stereochemistry , enzyme , nanotechnology , gene , biochemistry , materials science , engineering , mechanical engineering
The inside back cover picture shows the reconstruction of the ancestral sequence of haloalkane dehalogenases. Ancestral enzymes exhibited significantly enhanced thermodynamic stability compared to extant ones, as well as higher specific activities with a preference for short, multisubstituted halogenated substrates. Ancestral sequence reconstruction represents a powerful strategy for constructing highly stable and active catalysts that can further serve as robust templates for directed evolution. More information can be found in the full paper by R. Chaloupkova, J. Damborsky, et al. on page 1448 in Issue 14, 2017 (DOI: 10.1002/cbic.201700197).