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FLUORO/NO: A Nitric Oxide Donor with a Fluorescence Reporter
Author(s) -
Ravikumar Govindan,
Bagheri Meisam,
Saini Deepak Kumar,
Chakrapani Harinath
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700155
Subject(s) - nitric oxide , fluorescence , signalling , chemistry , biophysics , combinatorial chemistry , esterase , nanotechnology , biochemistry , microbiology and biotechnology , computational biology , enzyme , biology , materials science , organic chemistry , physics , quantum mechanics
Nitric oxide (NO) plays significant signalling roles in cells; the controlled generation of NO is of therapeutic relevance. Although a number of methods for the delivery and detection of NO are available, these events are typically mutually exclusive. Furthermore, the efficiency of delivery of NO can be compromised by detection technologies that consume NO. Here, we report FLUORO/NO, an esterase‐activated diazeniumdiolate‐based NO donor with an in‐built fluorescence reporter. We demonstrate that this compound is capable of enhancing NO within cells in a dose‐dependent manner, accompanied by a similar increase in fluorescence. The compatibility of this tool to study NO‐mediated signalling as well as NO‐mediated stress is demonstrated. FLUORO/NO is a convenient tool that shows NO‐like activity and allows monitoring of NO release. This tool will help interrogate the redox biology of NO.