A Tandem Enzymatic sp 2 ‐C‐Methylation Process: Coupling in Situ S‐Adenosyl‐ l ‐Methionine Formation with Methyl Transfer | Zendy

Sadler Joanna C. | Zendy; Humphreys Luke D. | Zendy; Snajdrova Radka | Zendy; Burley Glenn A. | Zendy

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A Tandem Enzymatic sp 2 ‐C‐Methylation Process: Coupling in Situ S‐Adenosyl‐ l ‐Methionine Formation with Methyl Transfer

Author(s) -

Sadler Joanna C.,

Humphreys Luke D.,

Snajdrova Radka,

Burley Glenn A.

Publication year - 2017

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201700115

Subject(s) - methylation , tandem , methyltransferase , chemistry , in situ , alkylation , methionine , enzyme , stereochemistry , streptomyces , organic chemistry , biochemistry , bacteria , dna , amino acid , catalysis , biology , materials science , composite material , genetics

A one‐pot, two‐step biocatalytic platform for the regiospecfic C‐methylation and C‐ethylation of aromatic substrates is described. The tandem process utilises SalL ( Salinospora tropica ) for in situ synthesis of S ‐adenosyl‐ l ‐methionine (SAM), followed by alkylation of aromatic substrates by the C‐methyltransferase NovO ( Streptomyces spheroides ). The application of this methodology is demonstrated for the regiospecific labelling of aromatic substrates by the transfer of methyl, ethyl and isotopically labelled 13 CH 3, 13 CD 3 and CD 3 groups from their corresponding SAM analogues formed in situ.

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