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Carbohydrate Analogue Microarrays for Identification of Lectin‐Selective Ligands
Author(s) -
Hyun Ji Young,
Park Cheol Wan,
Liu Yanna,
Kwon Daeun,
Park SeongHyun,
Park Sungjin,
Pai Jaeyoung,
Shin Injae
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700091
Subject(s) - wheat germ agglutinin , lectin , disaccharide , chemistry , microarray , dna microarray , biochemistry , c type lectin , ligand (biochemistry) , microbiology and biotechnology , biology , gene , gene expression , receptor
Fifty‐five mono‐ and disaccharide analogues were prepared and used for the construction of microarrays to uncover lectin‐selective ligands. The microarray study showed that two disaccharide analogues, 28′ and 44′ , selectively bind to Solanum tuberosum lectin (STL) and wheat germ agglutinin (WGA), respectively. Cell studies indicated that 28′ and 44′ selectively block the binding of STL and WGA to mammalian cells, unlike the natural ligand LacNAc, which suppresses binding of both STL and WGA to cells.