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The 5′‐AG 5 CC‐3′ Fragment from the Human CPEB3 Ribozyme Forms an Ultrastable Parallel RNA G‐Quadruplex
Author(s) -
Kulikov Katharina,
Nozinovic Senada,
KathSchorr Stephanie
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700028
Subject(s) - ribozyme , ligase ribozyme , vs ribozyme , hairpin ribozyme , chemistry , porphyrin , g quadruplex , rna , stereochemistry , nuclear magnetic resonance spectroscopy , circular dichroism , crystallography , dna , biochemistry , gene
An unusually thermostable G‐quadruplex is formed by a sequence fragment of a naturally occurring ribozyme, the human CPEB3 ribozyme. Strong evidence is provided for the formation of a uniquely stable intermolecular G‐quadruplex structure consisting of five tetrad layers, by using CD spectroscopy, UV melting curves, 2D NMR spectroscopy, and gel shift analysis. The cationic porphyrin TMPyP4 destabilizes the complex.