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Synthetic Biomarker Design by Using Analyte‐Responsive Acetaminophen
Author(s) -
Nishihara Tatsuya,
Inoue Joe,
Tabata Sho,
Murakami Shinnosuke,
Ishikawa Takamasa,
Saito Natsumi,
Fukuda Shinji,
Tomita Masaru,
Soga Tomoyoshi
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700023
Subject(s) - acetaminophen , analyte , hydrogen peroxide , chemistry , in vivo , biomarker , combinatorial chemistry , pharmacology , chromatography , biochemistry , medicine , biology , microbiology and biotechnology
The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte‐responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof‐of‐concept, we designed hydrogen peroxide (H 2 O 2 )‐responsive APAP (HR‐APAP) and succeeded in H 2 O 2 detection with cellular and animal experiments. In fact, for blood samples following HR‐APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR‐APAP] accurately reflects in vivo differences in H 2 O 2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.