Premium
Synthetic Biomarker Design by Using Analyte‐Responsive Acetaminophen
Author(s) -
Nishihara Tatsuya,
Inoue Joe,
Tabata Sho,
Murakami Shinnosuke,
Ishikawa Takamasa,
Saito Natsumi,
Fukuda Shinji,
Tomita Masaru,
Soga Tomoyoshi
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700023
Subject(s) - acetaminophen , analyte , hydrogen peroxide , chemistry , in vivo , biomarker , combinatorial chemistry , pharmacology , chromatography , biochemistry , medicine , biology , microbiology and biotechnology
The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte‐responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof‐of‐concept, we designed hydrogen peroxide (H 2 O 2 )‐responsive APAP (HR‐APAP) and succeeded in H 2 O 2 detection with cellular and animal experiments. In fact, for blood samples following HR‐APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR‐APAP] accurately reflects in vivo differences in H 2 O 2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom