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The Cell‐Surface N‐Glycome of Human Embryonic Stem Cells and Differentiated Hepatic Cells thereof
Author(s) -
Montacir Houda,
Freyer Nora,
Knöspel Fanny,
Urbaniak Thomas,
Dedova Tereza,
Berger Markus,
Damm Georg,
Tauber Rudolf,
Zeilinger Katrin,
Blanchard Véronique
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201700001
Subject(s) - glycome , embryonic stem cell , embryoid body , glycan , microbiology and biotechnology , biology , glycomics , induced pluripotent stem cell , stem cell , cell , cellular differentiation , chemistry , biochemistry , glycoprotein , gene
Abstract Human embryonic stem cells (hESCs) are pluripotent stem cells that offer a wide range of applications in regenerative medicine. In addition, they have been proposed as an appropriate alternative source of hepatocytes. In this work, hESCs were differentiated into definitive endodermal cells (DECs), followed by maturation into hepatocyte‐like cells (HLCs). Their cell‐surface N‐glycome was profiled and also compared with that of primary human hepatocytes (PHHs). Undifferentiated hESCs contained large amounts of high‐mannose N‐glycans. In contrast, complex‐type N‐glycans such as asialylated or monosialylated biantennary and triantennary N‐glycans were dominant in HLCs, and fully galactosylated structures were significantly more abundant than in undifferentiated hESCs. The cell‐surface N‐glycosylation of PHHs was more biologically processed than that of HLCs, with bisialylated biantennary and trisialylated triantennary structures predominant. This is the first report of the cell surface N‐glycome of PHHs and of HLCs being directly generated from hESCs without embryoid body formation.