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OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation
Author(s) -
Hughes Ryan R.,
Shaaban Khaled A.,
Zhang Jianjun,
Cao Hongnan,
Phillips George N.,
Thorson Jon S.
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600676
Subject(s) - glycosyltransferase , glycosylation , glycosyl , amine gas treating , chemistry , catalysis , substrate (aquarium) , combinatorial chemistry , tertiary amine , glycan , biochemistry , organic chemistry , enzyme , biology , ecology , glycoprotein
Abstract We describe the ability of an engineered glycosyltransferase (OleD Loki) to catalyze the N‐glycosylation of tertiary‐amine‐containing drugs and trichostatin hydroxamate glycosyl ester formation. As such, this study highlights the first bacterial model catalyst for tertiary‐amine N‐glycosylation and further expands the substrate scope and synthetic potential of engineered OleDs. In addition, this work could open the door to the discovery of similar capabilities among other permissive bacterial glycosyltransferases.