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Light‐Activated Triazabutadienes for the Modification of a Viral Surface
Author(s) -
Jensen Stephanie M.,
Kimani Flora W.,
Jewett John C.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600508
Subject(s) - surface modification , scaffold , chemistry , dengue virus , click chemistry , viral envelope , posttranslational modification , aryl , combinatorial chemistry , biophysics , nanotechnology , virus , biochemistry , materials science , biology , glycoprotein , virology , organic chemistry , computer science , alkyl , database , enzyme
Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host–pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.