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A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons
Author(s) -
Kim Beomsue,
Feng Suihan,
Yun SeongWook,
Leong Cheryl,
Satapathy Rudrakanta,
Wan Si Yan Diana,
Chang YoungTae
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600490
Subject(s) - progenitor cell , neural stem cell , fluorescence , progenitor , stem cell , chemistry , cellular differentiation , microbiology and biotechnology , fluorescent protein , neuroscience , biology , green fluorescent protein , biochemistry , physics , gene , quantum mechanics
Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13‐stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2 low cells from heterogeneous cell populations.