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Cover Picture: Generation of New Complestatin Analogues by Heterologous Expression of the Complestatin Biosynthetic Gene Cluster from Streptomyces chartreusis AN1542 (ChemBioChem 18/2016)
Author(s) -
Park OkKyung,
Choi HaYoung,
Kim GeonWoo,
Kim WonGon
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600457
Subject(s) - cosmid , heterologous expression , gene cluster , heterologous , gene , biology , streptomyces , genetics , microbiology and biotechnology , recombinant dna , bacteria
The cover picture shows the reconstitution of the 54.5‐kb complestatin biosynthetic gene cluster from two cosmids with a 16.5‐kb overlap into a single cosmid by λ‐RED recombination‐mediated assembly. Heterologous expression of the assembled gene cluster in Streptomyces lividans TK24 resulted in the production of complestatin. Deletion of cytochrome P450 monooxygenase genes (open reading frames 10 and 11) and heterologous expression of the modified gene clusters led to the production of two new monocyclic and linear derivatives, complestatins M55 and S56. The cytochrome P450 monoxygenases catalyze the cyclization of compleatstin in a strict order. More information can be found in the full paper by W.‐G. Kim et al. on page 1725 in Issue 18, 2016 (DOI: 10.1002/cbic.201600241).