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DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity
Author(s) -
Cox Amanda J.,
Bengtson Hillary N.,
Gerasimova Yulia V.,
Rohde Kyle H.,
Kolpashchikov Dmitry M.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600438
Subject(s) - deoxyribozyme , biosensor , sensitivity (control systems) , substrate (aquarium) , detection limit , catalysis , chemistry , nanotechnology , diffusion , biophysics , materials science , biochemistry , biology , chromatography , physics , ecology , engineering , electronic engineering , thermodynamics
Some natural enzymes increase the rate of diffusion‐limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three‐ to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion‐limited enzymes and to improve sensitivity of enzyme‐based biosensors.