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Cover Picture: G‐Quadruplexes with Tetra(ethylene glycol)‐Modified Deoxythymidines are Resistant to Nucleases and Inhibit HIV‐1 Reverse Transcriptase (ChemBioChem 15/2016)
Author(s) -
TateishiKarimata Hisae,
Muraoka Takahiro,
Kinbara Kazushi,
Sugimoto Naoki
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600390
Subject(s) - oligonucleotide , reverse transcriptase , dna , chemistry , ethylene glycol , g quadruplex , combinatorial chemistry , duplex (building) , human immunodeficiency virus (hiv) , microbiology and biotechnology , rna , biology , biochemistry , virology , organic chemistry , gene
The cover picture shows a G‐triplex‐forming oligonucleotide with tetra(ethylene glycol) (TEG)‐modified deoxythymidines. G‐quadruplex formation in virally encoded templates arrests reverse transcription, so methods to stabilize this structure are promising for antiviral approaches. To stabilize G‐quadruplex formation, deoxythymidines were modified with TEG. The TEG‐modified G‐quadruplexes were significantly stabilized relative to unmodified DNA. In the presence of a TEG‐modified oligonucleotide that can form an intermolecular G‐quadruplex with a template containing a human immunodeficiency virus‐1 sequence, reverse transcription was inhibited by more than 70 % relative to the reaction in the absence of the TEG‐modified oligonucleotide. Moreover, the TEG‐modified deoxythymidines protected the DNA oligonucleotide from degradation by various nucleases in human serum. Thus, DNA oligonucleotides modified with TEG have potential therapeutic applications. More information can be found in the communication by N. Sugimoto et al. on page 1399 in Issue 15, 2016 (DOI: 10.1002/cbic.201600162).