Premium
Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif
Author(s) -
BereczkTompa Éva,
Pósfai Mihály,
Tóth Balázs,
Vonderviszt Ferenc
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600377
Subject(s) - flagellin , magnetite , magnetotactic bacteria , protein filament , magnetite nanoparticles , biophysics , magnetosome , chemistry , mutant , template , nanotechnology , materials science , biology , biochemistry , magnetic nanoparticles , nanoparticle , receptor , gene , metallurgy
This work aimed at developing a novel method for fabricating 1 D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite‐binding motifs: two contained fragments of magnetosome‐associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop‐containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof‐of‐concept that flagellar filaments can be engineered to facilitate formation of 1 D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.