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CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells
Author(s) -
Rau Kristina,
Rentmeister Andrea
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600342
Subject(s) - crispr , cas9 , rna , guide rna , messenger rna , computational biology , synthetic biology , live cell imaging , microbiology and biotechnology , biology , chemistry , gene , cell , genetics
Cas9 can be implemented as an RNA‐targeting system to track mRNA in living cells. Nuclear export is enabled by efficient targeting of GFP‐fused Cas9 to an endogenous mRNA. The approach provides a new and versatile platform for RNA‐targeting with applications in RNA imaging and beyond.