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Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye‐Sensitized TiO 2
Author(s) -
Ainsworth Emma V.,
Lockwood Colin W. J.,
White Gaye F.,
Hwang Ee Taek,
Sakai Tsubasa,
Gross Manuela A.,
Richardson David J.,
Clarke Thomas A.,
Jeuken Lars J. C.,
Reisner Erwin,
Butt Julea N.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600339
Subject(s) - shewanella oneidensis , proflavine , flavin mononucleotide , chemistry , electron transfer , shewanella , flavin adenine dinucleotide , photochemistry , cytochrome b6f complex , flavin group , biophysics , photosystem i , cofactor , biochemistry , photosystem ii , bacteria , biology , photosynthesis , dna , genetics , enzyme
Abstract The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar‐assisted chemical synthesis. In Shewanella oneidensis MR‐1 (MR‐1), trans‐outer‐membrane electron transfer is performed by the extracellular cytochromes MtrC and OmcA acting together with the outer‐membrane‐spanning porin ⋅ cytochrome complex (MtrAB). Here we demonstrate photoreduction of solutions of MtrC, OmcA, and the MtrCAB complex by soluble photosensitizers: namely, eosin Y, fluorescein, proflavine, flavin, and adenine dinucleotide, as well as by riboflavin and flavin mononucleotide, two compounds secreted by MR‐1. We show photoreduction of MtrC and OmcA adsorbed on Ru II ‐dye‐sensitized TiO 2 nanoparticles and that these protein‐coated particles perform photocatalytic reduction of solutions of MtrC, OmcA, and MtrCAB. These findings provide a framework for informed development of strategies for using the outer‐membrane‐associated cytochromes of MR‐1 for solar‐driven microbial synthesis in natural and engineered bacteria.